PDF | The present study aims to evaluate the therapeutic properties of the medicinal plants (Vitex negundo, Aegle marmelos and Boswellia serrata) for their . Abstract To evaluate the anti-inflammatory activity of Vitex negundo (VN) leaf extract and its mechanism of action in experimental animals. Carrageenin induced. Abstract. The present study was undertaken to assess the anti-inflammatory effect of ethanolic extract of Vitex negundo roots in rats.

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Ferrous sulphate, ascorbic acid, sodium chloride, potassium chloride, all of LR grade were obtained from local suppliers. To this reaction mixture thiobarbituric acid was added and the mixture was boiled for 15 min. Pergamon Press, New York, pp: The experiment was repeated for ten times.

The animals from cotton granuloma test were used in four groups with ten animals in each group. Ethanolic leaf extract of VN in the form of suspension was fed orally in a volume of 10 mL kg -1 wt. D I Toxicant control Carrageenan 0. Herbal drugs play an important role in health care programs especially in developing countries.

Histomorphological changes induced by Vitex negundo in albino rats.

The inflammagory drug showed significant anti-inflammatory activity in dose dependant manner in both experimental models. This article has been cited by other articles in PMC.

Support Center Support Center. The difference in wet and dry weights of granuloma from control group to that of treated group indicates the anti-inflammatory activity.

Absorbance of the resulting pink chromogen was recorded at nm.


Antioxidant and Antiinflammatory Activity of Vitex negundo.

Studies on analgesic and anti inflammatory activity of Vitex negundo Linn. Group V received phenylbutazone mg kg -1 orally and Ibuprofen mg kg -1 B.

The present work was undertaken to evaluate the antioxidant potential and anti-inflammatory activity of the plant. Kundnani College of Pharmacy. These observations suggest that VN possesses anti-inflammatory activity against acute as well as sub-acute inflammation, which appear to be due to prostaglandin inhibition and reduction of oxidative stress respectively, which needs to be substantiated by further study.

It is concluded that hydroalcoholic leaves extract of Vitex negundo and Murraya koenigii can offer protection against carrageenan induced paw edema in rats. J Agric Food Chem. Ascorbic acid was negkndo as positive control. Leaves are applied externally to bruises and vitwx [ 9 ]. It is well established that oxytocin induced uterine contractions are partly mediated through prostaglandins Rall, X 7 days, p. Wiley Series Chemistry and Pharmacology snti Drugs.

Antioxidant and Antiinflammatory Activity of Vitex negundo

After 7 days drug treatment in cotton pellet granuloma method, mL of blood was collected from inner canthus of eye from each animal using capillary tube, in a vial containing EDTA as an anticoagulant.

J Lab Clin Med. Oxygen toxicity and antioxidants: The plant was collected from local area of Sevagram in the month of June, Necessary permission was obtained from the Institutional Animal Ethics Committee. The pastes of leaves are applied externally to treat the bites of poisonous animals [ 7 ]. Flavonoids as anti-oxidants evaluated by in vitro and amti situ liver chemiluminescence.

The liver was weighed and perfused with 0. The oxidative stress is the condition where Reactive Oxygen Species ROS generation exceeds endogenous anti-oxidant defense Sen, and it is well known that in chronic and sub-acute inflammation reactive oxygen species play an important role in modulating the extent of inflammatory response and consequent tissue and cell injury Robbin et al.


For mechanism of anti-inflammatory action, VN in the dose mg kg -1p. Thirty minutes after drug or test compound extracts administration, 0. Albino mice of either sex, weighing gm were used to determine the lethal dose. Please review our privacy policy. An infusion of the toasted leaves in used to stop vomiting [ 6 ]. The same procedure was repeated at 30 min 1, 2, 3 h.

C Harinath, Director and Head J. Suspension of diclofenac sodium 6. In cotton pellet granuloma test VN extract in graded dosesand mg kg Carrageenan-induced paw edema being an in-vivo investigational model for acute inflammation which been extensively used to determine the anti-inflammatory effect of new investigational agents. Footnotes Kulkarni, et al.: The clearance for the use of animals for experimental purpose was obtained knflammatory institutional ethical committee constituted for the purpose at M.

Plasma was separated by centrifugation at rmp for 10 min. Livingstone, Edinburgh and London. The activity was evaluated negunndo using carrageenan induced paw edema in rats.